Journal: Cell reports

Article Title: Low-intensity pulsed ultrasound modulates disease progression in the SOD1 G93A mouse model of amyotrophic lateral sclerosis.

doi: 10.1016/j.celrep.2024.114660

Figure Lengend Snippet: Figure 4. Effects of LIPUS on CBF and endothelial cell integrity in SOD1G93A mice (A) Representative CBF maps generated from perfusion 9.4T MRI in four groups of mice at 120 days of age. (B) Quantification of CBF at the motor cortex. (C) Representative EM photomicrographs of capillaries in the motor cortex of four groups of mice; scale bar, 1 mm. En indicates endothelial cell; black asterisks indicate edema in endothelial cells; M indicates mitochondria. (D, F, and H) Representative western blotting of the expression of VE-cadherin and PDGFRb (D), MMP-9 (F), and eNOS (H). (E, G, and I) Quantification analysis of VE-cadherin and PDGFRb (E), MMP-9 (G), and eNOS (I) expression in the motor cortex of four groups of mice; N = 5 in each group. Data are presented as the mean ± SD. The p values were determined by two-way ANOVA. **p < 0.01 compared with the Tg-NT group; ##p < 0.01 compared with the WT-NT group.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-VE-cadherin Cell Signaling Technology Cat#93467 anti-PDGFRb Proteintech Cat#13449-1-AP anti-MMP-9 Cell Signaling Technology Cat#3852S Cyt C Abcam Cat#ab133504 VEGFA Proteintech Cat#19003-1-AP BDNF Servicebio, China Cat#GB11559-100 anti-GAPDH Cell Signaling Technology Cat#5174 eNOS Servicebio Cat#GB115277-100 VE-Cadherin ABclonal Cat#A25533 IgG H&L(Alexa Fluor555) Abcam Cat#ab150078 Dapi Adamas life Cat#C8055 Chemicals, Peptides, and Recombinant Proteins FITC-dextran Sigma-Aldrich Cat#FD2000S Lipofectamine3000 Invitrogen Cat#L3000015 Cell Counting Kit-8 DOJINDO Cat#CK04 Dihydroethidium(DHE) YEASEN Cat#50102ES FITC-Dextran Adamas Cat#60842-46-8 DMEM Gibco N/A Opti-MEM Gibco Cat#31985070 Deposited data RNA-seq data and spatial transcriptomics data This study GEO Submission (GSE263117) Experimental Models: Cell Lines b.End3 Cell Bank of Chinese Academy of Sciences N/A C8-D1A Cell Bank of Chinese Academy of Sciences N/A Experimental models: Organisms/strains SOD1G93A mice Jackson Laboratory B6SJL-Tg-SOD1*G93A-1Gur/J, No. 002726 Oligonucleotides Primers for qRT-PCR This study Table S1 Piezo1 siRNA and TRPV4 siRNA This study Table S2 Software and algorithms MATLAB MathWorks,US https://ww2.mathworks.cn/ ImageJ National Institutes of Health https://imagej.net/Fiji custom-written MATLAB scripts This study https://doi.org/10.5281/zenodo.12742828 Other Hydrophone Precision Acoustics, UK NH2000 CatWalk system Noldus Information Technology CatWalk XT 10.6 multivariate system Gripping force meter Yiyan, China YLS-13A 9.4T MRI Bruker Bruker BioSpec 9.4T scanner Multiphoton laser-scanning microscope Olympus, Japan FV1200MPE 16 Cell Reports 43, 114660, September 24, 2024

Techniques: Generated, Western Blot, Expressing